Data tillgänglighet; Kompletterande information; PDF-filer; Kompletterande information A Millipore Multiscreen HTS-IP plate was coated overnight at 4 °C with LSR FORTESSA (BD Biosciences) was used for cell acquisition and data

FACSymphony A3 and BD FACSymphony A5 flow cytometers. The following list includes the available documentation for the system. •. BD FACSDiva Software

3. Turn on main power switch (Green button-right side) 4. † BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD® High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. † BD FACSFlow™ Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system. † A printed copy of the BD High Throughput Sampler User’s Guide is distributed with the BD™ High Throughput Sampler (HTS) option. This document describes how to set up and operate the HTS. It also contains a description of BD FACSDiva software features specific to the HTS. Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates.

Bd fortessa hts user guide

The HTS provides users the ability to acquire samples from 96-well or 384-well plates for efficient analysis with This guide describes how to set up and operate the BD™ High Throughput Sampler (HTS) option with the BD™ LSR II, BD FACSCanto™, and BD FACSCanto™ II flow cytometers. You should know how to operate your flow cytometer before using the HTS option. For important safety information, refer to the safety booklet provided with the HTS option. 6 BD LSRFortessa Cell Analyzer User’s Guide z BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: … FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn … to)running)an)HTS)experiment.)The)HTS)uses)more)sheath)fluid)than)loading) thetubes.) 2.

1. Switch on the LSR-Fortessa at the side of the instrument. This will warm up the lasers and pressurize the fluidics. 2. Switch on the Computer and login to the DiVa software. Even if you are using tubes instead of the HTS, you will have to wait for the HTS to go through its prime. 3. Check the fluidics. Fludics

HTS Throughput Acquisition: Less than 15 minutes per microtiter plate in high-throughput mode using a 2-second http://stratedigm.com offers affordable flow cytometer as an alternative to the BD LSR II & BD Fortessa.Call( 408) 512-3901 to learn more about our flow cyto BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility.

The BD™ High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate. The HTS can be front or side mounted on the BD LSRFortessa. HTS throughput Acquisition: Less than 15 minutes per microtiter plate in high throughput mode using a 2-second

Switch on the LSR-Fortessa at the side of the instrument. This will warm up the lasers and pressurize the fluidics. 2. Switch on the Computer and login to the DiVa software. Even if you are using tubes instead of the HTS, you will have to wait for the HTS to go through its prime. 3.

We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility.
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† BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD® High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. † BD FACSFlow™ Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system.

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BD LSR Fortessa X20 – How to Use Guide 4 3 At the top of the Browser panel, create a New Experiment (click the icon with a book). 4 Create a New Specimen (click the icon with a syringe). 5 Select Tube_001 (inside the syringe) by clicking in the green arrow. 6 Do 3 PRIMEs with the SIP 2 arm open and no tube installed. Then set the cytometer to

Remove the HTS safety cover. Locate and loosen the positioning screw that secures the HTS to the cytometer support bracket (Figure 4-17 on page 133). The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols.

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6 BD LSRFortessa Cell Analyzer User’s Guide z BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: …

2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment. 3) Click new plate and choose the plate type. University of Helsinki This Getting Started guide provides tutorials to familiarize you with BD FACSDiva™ software. If you are a new user, use the tutorials to get started with the software.

BD Biosciences does not make any warranty with respect to the workstation remaining virus free after installation. BD Biosciences is not liable for any claims related to or resulting from buyer/user's failure to install and maintain virus protection. BD FACSDiva software contains VxWorks as embedded software (“Run-Time Module”).

It is equipped with an HTS (High Throughput System) or plate reader This page is about BD FACSCanto HTS,contains Products Flow Cytometry,BD FACSCanto II Cytometry Core Facility,BD Bd Fortessa Hts User Guide. Instrument Guides. Work in progress. Quick Guides. Fortessa/LSRII; Attune NxT; BD FACS Aria BioLegend · BD Biosciences BD Accuri C6 BD LSR-II HTS. The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer. provides rapid, fully automated sample acquisition from microtiter BD Fortessa X20 is a state - of - the - art analyser equipped with 5 spatially This cytometer is equipped with HTS - high throughput sampler which allows you to load your samples directly from 96/384 wp.

Login to DIVA software. Before proceeding with the HTS, clean the instrument according to standard AMREPFLOW SOPs: • Check fluidics: empty waste and fill sheath. A while back, we were recommended away from routine PRIME functions for this very reason (BD service recommendation). Now, we PRIME only when we see a need – failures or problems with QC, data shifts within an experimental run, or samples fail to flow. We apply this principle to all our analyzers -- LSRII, Fortessa, Fortessa X-20 .